小鱼儿绿了 千种毒素现形

  • 日期:07-16
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胜博发娱乐

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在显微镜下,可以观察到暴露于有毒物质后鱼胚胎的变化。

扫描二维码,了解小鱼如何为您“测试”

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不同浓度的毒素可以产生不同的效果。

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鱼胚生物测定技术可以快速检测出数千种毒素。

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鱼胚生物测定技术可以快速检测出数千种毒素。

在古装剧中,你经常可以在葡萄酒茶餐中看到银针,针变成黑色,每个人都惊呼“有毒”的场景,而实际上,毒素的分析需要精确的化学检测,是否有直观和简单检查方法可以检测有毒物质吗?最近,在2019年中国化妆品国际高峰论坛上,记者获悉,使用绿色荧光蛋白标记技术,鲑鱼和斑马鱼可以快速检测出数千种毒素。鱼胚遇到有毒物质后,身体会发出绿色。荧光。据报道,这种可视化鱼胚生物检测技术正在准备用于越来越多的功效测试,如空气质量,美容美白,抗衰老和其他效果。

文:广州日报的全媒体记者刘丽琴

图文:广州日报全媒体记者苏俊杰

视频拍摄:广州日报的全媒体记者刘丽琴,苏俊杰

视频剪辑:广州日报全媒体记者郑毅

诺贝尔奖技术援助

第15代鱿鱼和斑马鱼的繁殖

In the only laboratory in Asia that has been certified to ISO 17025 and tested for toxicology in fish embryos, it has raised more than 10,000 fish. The fish that are raised in the laboratory of the Hong Kong Science and Technology Park in China are not ordinary fish. They are squid and zebrafish using green fluorescent protein labeling technology. They have been raised to the 15th since the birth of the first generation. generation. The laboratory determines whether a toxin is present in the test by observing whether the fish embryo encounters a test sample that emits green fluorescence.

Why do fish embryos change like this when they encounter toxins and harmful substances? Chen Xueping, a British and EU registered toxicologist and co-founder of Aquatic Silver & CTO, told reporters that there is a Nobel Prize-winning technology application Scientists Shimomura, Martin Charfi and Qian Yongjian discovered And the transformation of green fluorescent protein and won the Nobel Prize in Chemistry that year. Green Fluorescent Protein (GFP) is a protein of about 238 amino acids that excites from blue to ultraviolet light and emits green fluorescence. At the same time, the green fluorescent protein gene can be transferred into the genome of different species and continuously expressed in the offspring.

It is reported that the "fish embryo toxicology technology" originated in the State Key Laboratory of the City University of Hong Kong in China in 2001. It uses a technical means to fluorescently label a gene. After years of development, technology has been applied in many fields. For example, a gene that is very sensitive to chronic toxic substances that are prone to cause estrogens such as cancer, infertility, nervous disorders, obesity, etc. is labeled as a stable fish.

xx"The squid embryos labeled with the green fluorescent protein labeling technique can be tested for chronic toxicants. The reason for choosing this fish is that the carp embryos develop slowly, and can be hatched after 12 to 14 days. After hatching, they are more robust. Acute toxicants have little effect on them, but they can respond quickly to chronic toxicants," Chen Xueping said in an interview. "The effects of chronic toxicants on organisms may take several years to be discovered, while squid embryos only last 24 hours. It can be reacted. When the chronic toxic estrogen is detected, the fish liver will emit green fluorescence, and the intensity of the light can quantify the amount of toxic substances. In severe cases, the fish embryo will not emit green fluorescence and die directly." /p>

Zebrafish embryos can be tested for acute poisons. Because the development of zebrafish embryos and human embryo development are very similar, the similarity with human disease-related genes is 84%, and the development speed is fast. The zebrafish embryo development is similar to the 12-week state of human embryo development, so the test results Has an important reference. When using zebrafish for toxicity testing, the development of fish embryos will be abnormal when acute toxic substances are detected, and will die directly in severe cases. In addition, it should be noted that animal experiments have always been controversial. According to the EU Animal Protection Law, fish embryos are not animals, and are highly recommended by international scientists for animal testing. It is a new type of testing method.

Consumer product safety requires biometric data

At present, in the food, cosmetics and other industries, chemical detection methods are usually used. Traditional chemical analysis is very precise, but there are limitations. Chemical tests only test limited indicators due to price and time considerations, and only test targets and known chemicals to judge. It is compliant and the machine cannot fully tell you how high the toxicity level is. Chen Xueping said, "In this case, biological data is needed, and various consumer products that are closely related to everyone actually need biometric data."

xx这种生物识别数据的意义何在?例如,在食用油的检测中,通过比较十多种方式,利用斑马鱼胚胎做急性毒性试验方法,可以区分劣质油和好油,并对几十种油品进行盲测。执行。在两天内,你可以很快发现劣质油的急性毒性非常高。在某些浓度为30g/L的情况下,一半以上的鱼胚会死亡。如果油是好的,500g/L就不会有死亡。测试提供的数据表明劣质油对生物体有害。

陈雪平说,目前,化学测试可以显示哪些重金属,防腐剂和化学成分超标。可以检测和识别具体的要素和组成部分,为相关部门制定法律法规提供依据,并能够准确检查。在生活中,食品和化妆品可以通过许多过程和链接来到消费者手中。即使原材料是安全的,经过各种加工,如何确保安全仍是一个问题。通过对鱼胚的毒理学测试,可以得出关于产品是否具有毒性和毒性风险的直观结论。在对同类产品的数据进行比较时,我们可以看到产品的优缺点,并结合化学分析,可以准确地检测出哪些原料和成分存在问题。

作为一项原始技术,鱼胚胎检测的结果应该采用什么样的标准?陈雪说,由于技术的原创性,没有相关的评估标准。在建立检测方法和数据库的同时,要同时进行国内标准和国际标准的建设。

技术发展方向

扩展到功效检测

据介绍,目前通过鱼胚胎毒理检测技术根据科学数据,可以检测出一千多种慢性毒物、一千多种急性毒物。在急性毒物中有不少是防腐剂,乳化剂、香精等,一些化妆品中的慢性毒物中有化学防晒剂、香精,矿物油、植物原料等,这还涉及到纯度等问题。有个别的防腐剂、防晒剂在现有法规规定允许的浓度范围内还是不安全的,在慢性毒物检测过程中,鱼胚胎发出的绿色荧光会非常亮。陈雪平表示,技术发展的空间依然很大,目前因为鲭鱼和斑马鱼的独特性,在技术开发上还是围绕这两种鱼进行。技术团队已经建立过万种市面上销售产品的生物毒性数据库,但是全球各种商品种类繁多,要建立更大更全的数据库,还需要做大量的工作。而运用鱼胚胎检测技术,需要开发更多检测方法和手段,以获得更广泛的应用,目前功效检测正是发展方向之一。比如室内、汽车内空气质量的检测,化妆品美白祛斑、抗氧化、抗衰老等功效检测。

xxxx因为人们购买商品,他们不仅会考虑他们的安全,还会有功能要求。安全性有相应的监管标准,但基本上没有直观的功效评估标准,这需要更大量的数据积累。斑马鱼测试技术用于功效测试。研究表明,斑马鱼与人体心血管系统的一致性为95%,斑马鱼的皮肤颜色调节机制与人体肤色相同,而斑马鱼的研究结果和调节机制与人眼相同。因此,根据斑马鱼胚胎色素抑制率,可以评价化妆品的美白效果,根据斑马鱼胚胎的ROS清除率评价产品的抗氧化活性,并对斑马鱼进行尾鳍切除。胚胎。尾鳍斑马鱼的炎症细胞抑制率被评定为产品的抗炎作用,并且根据尾鳍斑马鱼尾鳍再生的比率评定产品的修复功效。通过从8小时工作和生活空间的人们收集空气样本到水中,并观察水中鱼胚胎的状态来测量空气质量。

资本援助技术

检验行业的目标是消费品市场

2018年10月,国家统计局发布《战略性新兴产业分类(2018)》,国家战略性新兴产业数量从7个增加到9个,检验认证和标准化服务成为新的战略性新兴产业。随着经贸发展水平的提高,工业,医药,食品等产品的质量安全要求不断提高。国家政策逐步放开,第三方检测行业于2014年正式进入市场化阶段,民营资本进入检验检测行业。根据国家认证认可监督管理局的数据,中国的检验和测试市场继续快速发展。目前,检验检测机构近4万家,每年发布检验报告4亿多份。业内人士表示,从测试的角度来看,传统工业测试仍然占据了一半以上的市场份额。然而,在消费升级的推动下,生命科学,环境,消费品测试,服务和系统认证等领域正在崛起。

在技术支持下,生物检测技术被用作传统物理和化学成分检测的补充,针对消费品市场,如食品,护肤化妆品,保健品,母婴用品等,小玉秦测科技有限公司在广州成立,为消费者提供安全保障。产品信息和测试报告。同时,它也引起了资本市场的关注,并获得了普华永道,南丰集团和湘江集团等众多资本的投资。 “作为普华永道首次投资生物技术和互联网平台,普华永道的战略选择是利用其检测技术的独特性和科学性。它还认为市场需要这样一个系统来增强其消费品的安全性和信任度, “水下银与联合创始人陈子祥说。

据了解,鱼胚技术已被用于国际药物筛选和测试。 2016年至2017年,资本进入主要用于技术再开发,作为第一个将此技术用于日常快速移动商品测试。该公司需要在预处理技术,测试过程,数据库建立和专利方面进行大量投资。通过独特的技术,产生的数据越多,就越能够使相关部门和消费者能够为供应链提供数据。据悉,今年该团队将联合普华永道和香港城市大学发布全球首个消费品安全指数。陈子祥说,目前的指标与消费品安全无关。有了该指数,就有了评级的基础。它还将在金融市场中得到相应的参考。显然,随着资本的不断发展,技术可以进一步发展。